Journal: The Journal of Neuroscience

Article Title: miR126-5p Downregulation Facilitates Axon Degeneration and NMJ Disruption via a Non–Cell-Autonomous Mechanism in ALS

doi: 10.1523/JNEUROSCI.3037-17.2018

Figure Lengend Snippet: miR126-5p is depleted in SOD1 G93A muscles and regulates Sema3 and NRP expression. A , NanoString chip screen heat map of significantly altered miRs in P60 muscles of SOD1 G93A compared with LM mice (extended table under ). Red and green represent a high or low abundance of miRs, respectively. * p < 0.05 (Student's t test; n = 3). B , miR126-5p was the most significantly downregulated miRNA in SOD1 G93A muscles (Student's t test; n = 3, ** p = 0.003). C , qPCR analysis of P60 GC muscle extracts further validates the decrease in miR 126-5p in SOD1 G93A ( n = 3). D–G , qPCR analysis of Sema3A, NRP1, Sema3B, and NRP2 transcript levels in HeLa cells overexpressing either miR126-5p or miR142 demonstrates a reduction in their expression levels specifically under miR126-5p overexpression (Student's t test; n = 3, * p = 0.0438, * p = 0.034, * p = 0.031, and * p = 0.0434, respectively). H , Representative TIRF images of U87MG cells reveal a detachment of the cell membrane from the culture dish surface after Sema3A is added to the culture medium. Scale bar, 10 μm. I , Impedance recording of live cells over time shows that U87MG cells overexpressing miR126-5p are unresponsive to Sema3A added to the culture medium because their impedance continuously increases, whereas the impedance of U87MG cells overexpressing miR142 decreases after treatment .

Article Snippet: Antibodies were used at the following concentrations: anti-Neurofilament Heavy Chain 1:500 (NFH, Abcam, ab72996; 1:1000), synaptophysin (Millipore, MAB5258; 1:300), synaptotagmin (Alomone Labs, ant-003; 1:300), anti-NRP1 (1:100), anti-Sema3A (1:100), anti-NRP2 (1:100), and anti-Sema3B (1:100).

Techniques: Expressing, Over Expression

Journal: Cancer Medicine

Article Title: Prediction of cervical lymph node metastasis from immunostained specimens of tongue cancer using a multilayer perceptron neural network

doi: 10.1002/cam4.5343

Figure Lengend Snippet: Results of classification by MNN. The results of the automatic classification of IHC specimens into high and low groups based on MNN and the number of patients in these groups with cervical lymph node metastasis (N+) and without (N−)

Article Snippet: The sections were incubated with the following primary antibodies at 4°C overnight: anti‐human rabbit polyclonal VEGF‐C antibody (dilution 1:100), anti‐human rabbit polyclonal VEGF‐D antibody (dilution 1:200) (both from Abcam), anti‐human goat polyclonal CCR7 antibody (dilution 1:250), anti‐human rabbit monoclonal NRP1 antibody (dilution 1:100) (both from Abcam), anti‐human rabbit polyclonal NRP2 antibody (dilution 1:100; Atlas Antibodies), and anti‐human goat polyclonal SEMA3E antibody (dilution 1:100; Abcam).

Techniques:

Journal: Cancer Medicine

Article Title: Prediction of cervical lymph node metastasis from immunostained specimens of tongue cancer using a multilayer perceptron neural network

doi: 10.1002/cam4.5343

Figure Lengend Snippet: Association of risk factors with cervical lymph node metastasis based on the results of the automatic classification with MNN. The risk factor is cervical lymph node metastasis

Article Snippet: The sections were incubated with the following primary antibodies at 4°C overnight: anti‐human rabbit polyclonal VEGF‐C antibody (dilution 1:100), anti‐human rabbit polyclonal VEGF‐D antibody (dilution 1:200) (both from Abcam), anti‐human goat polyclonal CCR7 antibody (dilution 1:250), anti‐human rabbit monoclonal NRP1 antibody (dilution 1:100) (both from Abcam), anti‐human rabbit polyclonal NRP2 antibody (dilution 1:100; Atlas Antibodies), and anti‐human goat polyclonal SEMA3E antibody (dilution 1:100; Abcam).

Techniques:

Journal: Cancer Medicine

Article Title: Prediction of cervical lymph node metastasis from immunostained specimens of tongue cancer using a multilayer perceptron neural network

doi: 10.1002/cam4.5343

Figure Lengend Snippet: Results of classification by HS histogram. The results of the automatic classification of IHC specimens into high and low groups based on HS histogram and the number of patients in the group with cervical lymph node metastasis (N+) and without (N−)

Article Snippet: The sections were incubated with the following primary antibodies at 4°C overnight: anti‐human rabbit polyclonal VEGF‐C antibody (dilution 1:100), anti‐human rabbit polyclonal VEGF‐D antibody (dilution 1:200) (both from Abcam), anti‐human goat polyclonal CCR7 antibody (dilution 1:250), anti‐human rabbit monoclonal NRP1 antibody (dilution 1:100) (both from Abcam), anti‐human rabbit polyclonal NRP2 antibody (dilution 1:100; Atlas Antibodies), and anti‐human goat polyclonal SEMA3E antibody (dilution 1:100; Abcam).

Techniques:

Journal: Cancer Medicine

Article Title: Prediction of cervical lymph node metastasis from immunostained specimens of tongue cancer using a multilayer perceptron neural network

doi: 10.1002/cam4.5343

Figure Lengend Snippet: Association of risk factors with cervical lymph node metastasis based on the results of the HS histogram analysis. Risk factor is cervical lymph node metastasis

Article Snippet: The sections were incubated with the following primary antibodies at 4°C overnight: anti‐human rabbit polyclonal VEGF‐C antibody (dilution 1:100), anti‐human rabbit polyclonal VEGF‐D antibody (dilution 1:200) (both from Abcam), anti‐human goat polyclonal CCR7 antibody (dilution 1:250), anti‐human rabbit monoclonal NRP1 antibody (dilution 1:100) (both from Abcam), anti‐human rabbit polyclonal NRP2 antibody (dilution 1:100; Atlas Antibodies), and anti‐human goat polyclonal SEMA3E antibody (dilution 1:100; Abcam).

Techniques: